rabbit anti-bmp2 antibody (ABclonal Biotechnology)
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90
Structured Review
ABclonal Biotechnology
rabbit anti-bmp2 antibody
Rabbit Anti Bmp2 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-bmp2+antibody/pmc10931601-184-36-40?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
Rabbit Anti Bmp2 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-bmp2+antibody/pmc10931601-184-36-40?v=ABclonal+Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-bmp2 antibody - by Bioz Stars,
2026-07
90/100 stars
Images
1) Product Images from "Proanthocyanidins Ameliorate LPS-Inhibited Osteogenesis of PDLSCs by Restoring Lysine Lactylation"
Article Title: Proanthocyanidins Ameliorate LPS-Inhibited Osteogenesis of PDLSCs by Restoring Lysine Lactylation
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms25052947
Figure Legend Snippet: Osteogenesis and lactylation levels are decreased in inflamed periodontal tissue and LPS-stimulated PDLSCs. ( A ) H&E staining in rat periodontal tissues and immunohistochemical Pan Kla and RUNX2 expression. ( B ) Quantitative analysis of positive areas. ( C ) Western blotting showing protein levels of COL-1, ALP, RUNX2, and BMP2 in LPS-treated PDLSCs. ( D ) qRT-PCR showing mRNA levels of osteogenic genes COL-1 , ALP, RUNX2 , and BMP2 . ( E ) ALP staining at 7 days and ARS staining at 21 days of osteogenic induction in LPS-treated PDLSCs. ( F ) Lactate concentration in the culture medium of PDLSCs was measured at the indicated times, as shown in the quantitative analysis. ( G ) Western blotting showing the protein level of Pan Kla in LPS-treated PDLSCs. ( H ) Quantitative analysis of Pan Kla protein levels. ( I ) Immunofluorescence staining images showing the expression levels of RUNX2 (green) and Pan Kla (red) in PDLSCs after LPS treatment. Cell nuclei were stained with DAPI (blue). PDLSCs: periodontal ligament stem cells; Kla: lysine lactylation; LPS: lipopolysaccharide; NC: healthy mice without periodontitis; H&E: hematoxylin and eosin staining (Data are expressed as mean ± SD, N = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS indicates no statistical difference).
Techniques Used: Staining, Immunohistochemical staining, Expressing, Western Blot, Quantitative RT-PCR, Concentration Assay, Immunofluorescence
Figure Legend Snippet: Restoration of lactylation by TSA recovers the osteogenesis of PDLSCs in an inflammatory state. ( A ) Western blotting showing the protein levels of COL-1, ALP, RUNX2, and BMP2 in PDLSCs after treatment with the conditions as shown. ( B ) Quantification of protein levels of COL-1, ALP, RUNX2, and BMP2. ( C ) qRT-PCR showing mRNA levels of osteogenic genes COL-1 , ALP, RUNX2, and BMP2 . ( D ) ALP staining at 7 days and ARS staining at 21 days of osteogenic induction in PDLSCs. ( E ) Quantitative analysis of ALP staining and ARS staining. ( F ) Immunofluorescence staining images showing the expression levels of RUNX2 (green) and Pan Kla (red) in PDLSCs treated with the conditions as shown. Cell nuclei were stained with DAPI (blue). ( G ) Pearson linear regression analysis of RUNX2 and Pan Ka fluorescence intensity. PDLSCs: periodontal ligament stem cells; Kla: lysine lactylation; LPS: lipopolysaccharide; TSA: Trichostatin A (Data are expressed as mean ± SD, N = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS indicates no statistical difference).
Techniques Used: Western Blot, Quantitative RT-PCR, Staining, Immunofluorescence, Expressing, Fluorescence
Figure Legend Snippet: Proanthocyanidins promote osteogenesis and elevate the lactylation of PDLSCs. ( A ) Western blotting showing protein levels of COL-1, ALP, RUNX2, and BMP2 in PA-treated PDLSCs. ( B ) Quantification of protein levels of COL-1, ALP, RUNX2, and BMP2. ( C ) qRT-PCR showing mRNA levels of osteogenic genes COL-1 , ALP, RUNX2 , and BMP2 . ( D ) ALP staining at 7 days and ARS staining at 21 days of osteogenic induction in PA-treated PDLSCs. ( E ) Lactate concentration in the culture medium of PDLSCs was measured at the indicated times as shown in the quantitative analysis. ( F ) Western blotting showing the protein level of Pan Kla in PA-treated PDLSCs. ( G ) Quantitative analysis of Pan Kla protein levels. ( H ) Immunofluorescence staining images showing the expression levels of RUNX2 (green) and Pan Kla (red) in PDLSCs after treatment with the conditions as shown. Cell nuclei were stained with DAPI (blue). PDLSCs: periodontal ligament stem cells; Kla: lysine lactylation; PA: proanthocyanidins (Data are expressed as mean ± SD, N = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS indicates no statistical difference).
Techniques Used: Western Blot, Quantitative RT-PCR, Staining, Concentration Assay, Immunofluorescence, Expressing
Figure Legend Snippet: Proanthocyanidins recover PDLSC osteogenesis by restoring the lactylation of PDLSCs in an inflammatory state. ( A ) Western blotting showing the protein levels of COL-1, ALP, RUNX2, and BMP2 in PDLSCs after treatment with the conditions as shown. ( B ) Quantification of protein levels of COL-1, ALP, RUNX2, and BMP2. ( C ) qRT-PCR showing mRNA levels of osteogenic genes COL-1 , ALP, RUNX2, and BMP2 . ( D ) ALP staining at 7 days and ARS staining at 21 days of osteogenic induction in PDLSCs. ( E ) Quantitative analysis of ALP staining and ARS staining. ( F ) Immunofluorescence staining images showing the expression levels of RUNX2 (green) and Pan Kla (red) in PDLSCs treated with the conditions as shown. Cell nuclei were stained with DAPI (blue). ( G ) Pearson linear regression analysis of RUNX2 and Pan Ka fluorescence intensity. PDLSCs: periodontal ligament stem cells; Kla: lysine lactylation; PA: proanthocyanidins; Theo-24: theophylline (Data are expressed as mean ± SD, N = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, NS indicates no statistical difference).
Techniques Used: Western Blot, Quantitative RT-PCR, Staining, Immunofluorescence, Expressing, Fluorescence
Figure Legend Snippet: Primer sequences.
Techniques Used: